- October 21, 2015 at 7:20 am #6867
Questions regarding normal peripheral blood go here.October 21, 2015 at 9:10 am #6890
Q: How many PBMCs can we get from one cyno?
A: 100ml of whole blood can be withdrawn without killing the animal.
Q: PB007 is a fresh leucopack of mononuclear cells plus 50 to 150mL of autologous plasma, correct? Can you make an estimate how many PBMC you have in one leucopac ?
A: Our Leuko Pak total volume is 300ml (150ml of cells and 150ml of plasma) plus 32ml of ACD-A Anticoagulant. The average number of PBMCs in our Leuko Pak is anywhere from 15-20 billion cells.
Q: What is the approximate number of PBMC’s one could isolate from a ¼ leuko pak?
A: The average number of PBMCS in ¼ pak is 5 billion cells.
Q: What is the BSL of mPB008F cell line?
A: It should be treated as BSL 1.
Q: What is the purity of PBMCs?
A: The purity of our normal healthy donor PBMCs from leuko paks is usually around 98-99%. The PBMCs from diseased whole blood Leukemia and diseased autoimmune is usually around 85-95%.
Q: A customer is asking me what the cell viability and quality are for our cord blood MNCs? What do we quality check and certify the sample to be?Ã‚Â
A: Fresh or frozen? The average viability of the fresh cells is usually around 95%, but we guarantee 90%. For the frozen, the viability can range from 85-95% post thaw and we guarantee 70%. In some cases, we do get gender and ethnicity info of the baby, but not always.
Q: Are the PBMCs that we get allodepleted (no T-cells)?
A: No, we don’t allodeplete T cells from PBMC for the mixed-donors product unless the customer specifically requests so. We can custom deplete T cell before pooling donors if thats what is requested.
Q: Do you have 350 million size normal PBMC as a frozen product?
A: We don’t have this as a current product. But we certainly can make it as a custom product.
Q: Do you sell “negatively selected” monocytes?
A: We can only do the positive isolation method.
Q: Once the blood is delivered, how long is it able to stay refrigerated for?
A: You should use the blood as soon as possible once you receive it.
Q: What is the difference between leuko pak and buffy coat?
A: Check out our page that explains the difference between leuko pak and buffy coat:
Q: Could you please provide me with some specs on your leuko paks (PN# PB007)? I’d like to know what a full bag includes in terms of cell #, volume, and maybe an average breakdown of the cell population. Any other pertinent info would also be appreciated
A: Our product # PB007 is a full apheresis collection of highly pure PBMCs which comes from 3x blood volumes/donor ( approximately 12-18 liters from a normal adult), which is achieved by connecting a donor to an apheresis machine that utilizes gravity and cell density to separate and enrich cells over a continuous period of 4 hours. The procedure collects about 200-250mL of cells sitting in minimal amount of plasma and about an additional 50-100mL of plasma, which is then put back at the end of collection, making the final product 300mL. Our standard regimen of anticoagulant is ACD-A, which is used at about 10% v/v. On the average we get about 15-20e9 PBMCs per collection, with a few donor variations as low as 10e9 and as high as 25e9. In the final product, the biggest reduction of cell population is that of granulocytes/PMNs and red blood cells, compared to percentages in equal volume of whole blood. The granulocytes and RBCS are reduced to about <5-10%. In the PBMCs collected, about 25-30% are CD4+ T lymphocytes, 15% are CD8+ T lymphocytes, 10-15% CD14+ monocytes, 7-9% CD19+ B lymphocytes, 3-5% CD56+ NK cells, 0.5-1% plasmacytoid DCs, and about 0.1-0.2% CD34+ HPCs. These are just some of the major subpopulations of interest. If you would like to know more by specific activity or developmental status please let us know and we can provide further information.
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