- October 21, 2015 at 7:21 am #6868
Questions about human primary cells and tissue go here.October 21, 2015 at 9:04 am #6885
Q: Do you have pulmonary artery smooth muscle cell?
A: Unfortunately we currently don’t have access to donors with pulmonary hypertension. Should we access this patient population in the future, we will be sure to let you know.
Q: Do you have frozen human CD14+ CD16+ monocytes?
A: No we do not. However, we can isolate these as a custom product.
Q: Do you have spleen cDNA?
A: We don’t get human spleens in-house and will need to source them externally. I’m pretty confident that our platform can generate good quality cDNA from good quality spleen samples.
Q: Can you provide mixtures of the BM cDNA samples or do I have to order each item from your inventory separately?
A: We would honestly prefer that you order the minimum unit from each lot if possible, and we will gladly mix the units from different lots for you if that’d help. It’s not as optimal for us to subtract a portion of a single unit because that’ll leave us with non-standard products.. Anyways, please feel free to discuss how you’d like the samples mixed and then we can perhaps work something out.
Q: Do you have Vas deferens Smooth muscle cell or a Vas deferens tissue and a seminal vesicle tissue?
A: I am sorry, but we do not have access to Smooth Muscle cells, Vas deferens, or seminal vesicle tissue.
Q: Does AllCells offer podocytes or visceral epithelial cells?
A: Sorry, we don’t have a source for podocytes or visceral epithelial cells.
Q: I am hoping to order human PBMCs. Do you have the protein content for these cells?
Q: Can you culture mast cells using CD34+ and CD133+?
A: It takes 6-8 weeks to complete the culture and the recovery is 4-5 million mature mast cells with purity of 90%. The only issue with this cells are they don’t freeze well in any known cryo solution (DMSO, glycerol, etc.). They completely lyse like RBC when frozen. We also don’t know how well they do during transport and the ideal temperature they should be shipped in. We need to test further before we can provide these cells to our customers.
Q: Do you have normal donor serum?
A: Human serum is not a regular item we routinely provide. We will need to consider your inquiry as a custom product.
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