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  • #6869
    AllCells
    AllCells
    Keymaster

    Post questions about cord blood.

    #6888
    AllCells
    AllCells
    Keymaster

    Q:How many vials were made from one donor umbilical cord? It says on your website that to get the MNC the cord blood is diluted with PBS and then density gradient separation techniques are used. If you are able to tell me- do the scientists/technicians use Ficol or a different reagent?
    A: From an average single-donor cord blood we can make about 2-3 units of the CB003F. And yes, for the density separation we use ficol-paque of 1.077g/mL density.

    Q: Do you provide HUVEC-001F at P1?
    A: We will no longer be providing HUVEC-001F at P1. Instead, we will be providing this cells at P0. The uncultured HUVEC cells are cryopreserved after they are isolated from the umbilical cord. Our QA department will only culture the cells up to P1 to determine the cell doubling time.

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