Leukopaks Guaranteed Cell Counts

Leukopaks

Guaranteed Cell Count

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Guaranteed Cell Counts

Get a guaranteed number of cells in every Leukopak, each and every time.

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Three Formats

Available in 10B, 5B, and 2.5B TNC for your convenience.

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Consistency

Adherence to quality standards ensure cells are of a high purity and viability.

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Characterized

Surface antigen marker expression and complete blood counts provided for every Leukopak.

The Challenge of Donor Variability

Leukopaks are an enriched leukapheresis product collected from the peripheral blood from a single donor, consisting of a large number of mononuclear cells (T cells, B cells, NK cells, and monocytes). Leukapheresis collections result in higher purity and significantly higher MNC content than from buffy coat collection. Obtaining a more pure and higher quantity of mononuclear cells (MNCs) is important for research and clinical uses such as cell-based assays (proliferation, T-cell activation, etc.), cell-therapy process development, bio-marker discovery, and more.

Challenges arise during leukapheresis as the inherent variability of available cells is donor-dependent. While some of this variability can be addressed through donor management, all donors are unique individuals, demographic differences like age, size, and gender can affect apheresis results, and ultimately, have negative impacts on your experimental workflow.

Lot to lot variablity.
The inherent variability present in the donor population is easily observed, as demonstrated by the total nuclear cell (TNC) counts across a sampling of 52 individual apheresis collections from AllCells’ donor facility.

Eliminate the Donor Variability

With AllCells’ new Guaranteed Cell-count Leukopak products, you can overcome these challenges, saving time, money, and resources. Obtain no less than the number of cells you need for your experimental workflow in as little as 3-days. Please note that lead times vary depending on the scope of your project and donor availability. Please contact us to begin a statement of work or to make an inquiry.

The Guaranteed Cell-count Leukopaks are filled to deliver no less than 10 Billion, 5 Billion, or 2.5 Billion cells at release. Not only are you guaranteed a certain number of cells in each Leukopak, but additional product characterization, such as complete blood count (CBC) and immunophenotyping (by flow cytometry) for key surface markers, is provided. AllCells’ Guaranteed Cell-count Leukopaks take the guesswork out of the equation by delivering a product that is standardized, cost-effective, and characterized, increasing the odds of a successful experiment and minimizing error and costly repetition.

Guaranteed Cell-count Leukopaks are collected from our on-site, IRB-approved bi-coastal collection facilities located in Alameda, California, and Quincy, Massachusetts from an extensive pool of healthy, consenting, and recallable donors. Mononuclear cell-rich peripheral blood is collected by leukapheresis, using the continuous flow Spectra Optia® Apheresis System into ACD-A anticoagulant following a standardized protocol. Guaranteed Cell-count Leukopaks adhere to strict quality standards to ensure the product is of the highest purity, viability, and quality.

Representative gating strategy.
Representative gating strategy for immunophenotyping results obtained through flow cytometric analysis of the different reported subpopulations.
Mean percentages of cell subpopulations in leukapheresis product types as measured by flow cytometry.
Mean percentages of cell subpopulations.

Guaranteed Cell Count Leukopak, 10B Cells (LP, FR, 10B)

To demonstrate the inherent variability present in the donor population, we sampled 52 individual apheresis collections from the AllCells donor facility and observed significant variability as measured by the total nuclear cell (TNC) counts.

Choosing the Guaranteed Cell Count Leukopak, 10B cells ensures that you receive the same number of cells regardless of the donor, guaranteeing a consistent, quality-controlled product every time, simplifying planning your procedure downstream.

Leukopaks: Enriched in White Blood Cells for Immunology

Fresh Leukopaks are a rich source of T cells, B cells, and other white blood cells, critical for use in immunological research, cell therapy-process development, and drug development. Their ability to provide insight into immunological mechanisms makes them an integral part of scientific and medical research. On average, the white blood cell content is 72.7%+- 8.2 in each Leukopak. Our leukapheresis process outperforms whole blood buffy coat collection by delivering a product with higher purity and significantly higher MNC content per volume, which is important for applications requiring a high yield and purity of cells.

Leukopak, 10 Billion Cells

Product Specifications

Parameter Specification
TNC count NLT 10B
% Viability >90%
5-Part Differential CBC report
Immunophenotyping report

Ordering Information

Catalog # Description Size Format Price
LP, FR, 10B Guaranteed cell count Leukopak 10 billion cells Fresh Please inquire
LP, FR, 5B Guaranteed cell count Leukopak 5 billion cells
LP, FR, 2.5B Guaranteed cell count Leukopak 2.5 billion cells

Request a quote for your next order of high quality human primary cells.

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