Total: 215 Publications

 

Cell Type

cDNA

Endothelial Cells

Leuko Pak

Lymphoid Cells

Mononuclear Cells

Myeloid Cells

Plasma

RNA

Stem/Progenitor Cells

Stromal & Mesenchymal Cells

Whole Bone Marrow

Whole Tissue

Tissue Type

Bone Marrow

Cord Blood

Dental

Diseased Bone Marrow

Diseased Peripheral Blood

Mobilized Peripheral Blood

Normal Peripheral Blood

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Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells

Mark A. DeWitt, Wendy Magis, Nicolas L. Bray, Tianjiao Wang, Jennifer R. Berman, Fabrizia Urbinati, Seok-Jin Heo, Therese Mitros, Denise P. Muñoz, Dario Boffelli, Donald B. Kohn, Mark C. Walters, Dana Carroll, David I. Martin, Jacob E. Corn

Genetic diseases of blood cells are prime candidates for treatment through ex vivo gene editing of CD34+ hematopoietic stem/progenitor cells (HSPCs), and a variety of technologies have been proposed to treat these disorders. Sickle cell disease (SCD) is a recessive genetic disorder caused by a single-nucleotide polymorphism in the b-globin gene (HBB). We optimize design and delivery parameters of a ribonucleoprotein (RNP) complex comprising Cas9 protein and unmodified single guide RNA, together with a single-stranded DNA oligonucleotide donor (ssODN), to enable efficient replacement of the SCD mutation in human HSPCs.

Source: Science Translational Medicine. 8, 360ra134 (2016) 12 October 2016

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Central memory CD4+ T cells are preferential targets of double infection by HIV-1

Aiman A. Haqqani , Samantha L. Marek, Jagadish Kumar, Miles Davenport, Heng Wang, and John C. Tilton

Template switching between two distinct HIV-1 RNA genomes during reverse transcription gives rise to recombinant viruses that greatly expand the genetic diversity of HIV-1 and have adverse implications for drug resistance, immune escape, and vaccine design. Virions with two distinct genomes are produced exclusively from cells infected with two or more viruses, or ‘doubly infected’ cells.

Source: Virology Journal 2015 12:184 Published: 11 November 2015

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Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells

Fernando A. Fierro, Adam J. O’Neal , Julie R. Beegle, Myra N. Chávez, Thomas R. Peavy, Roslyn R. Isseroff , and José T. Egaña

Here we describe human bone marrow-derived MSC seeded in Integra matrices, a common type of scaffold for dermal regeneration (SDR). We show and measured the distribution of MSC within the SDR, where cells clearly establish physical interactions with the scaffold, exhibiting constant metabolic activity for at least 15 days.

Source: Front Cell Dev Biol. 2015; 3: 68. Published online 2015 Oct 30

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Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens

Wei Liao, Gwen Jordaan, Phillipp Nham, Ryan T. Phan, Matteo Pelegrini, and Sanjai Sharma

To determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.

Source: BioMed Central, October 16, 2015

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Human Umbilical Cord Blood Cells Induce Neuroprotective Change in Gene Expression Profile in Neurons After Ischemia Through Activation of Akt Pathway

Shahaduzzaman, M. D.; Mehta, Vijay; Golden, Jason E.; Rowe, Derrick D.; Green, Suzanne; Tadinada, Ramya; Foran, Elspeth A.; Sanberg, Paul R.; Pennypacker, Keith R.; Willing, Alison E.

Human umbilical cord blood (HUCB) cell therapies have shown promising results in reducing brain infarct volume and most importantly in improving neurobehavioral function in rat permanent middle cerebral artery occlusion, a model of stroke. In this study, we examined the gene expression profile in neurons subjected to oxygen-glucose deprivation (OGD) with or without HUCB treatment and identified signaling pathways (Akt/MAPK) important in eliciting HUCB-mediated neuroprotective responses.

Source: Cell Transplantation, Volume 24, Number 4, 2015, pp. 721-735(15)

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Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium

Siti Aisyah Mohd Taha , Joanne Koh Su Ling , Nur Izyan Binti Azmi , Thirumulu Ponnuraj Kannan, Ahmad Azlina, Khairani Idah Mokhtar

To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).

Source: Braz. J. Oral Sci. vol.14 no.2 Piracicaba Apr./June 2015

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Identification of TL-Om1, an Adult T-Cell Leukemia (ATL) Cell Line, as Reference Material for Quantitative PCR for Human T-Lymphotropic Virus 1

Madoka Kuramitsu, Kazu Okuma, Makoto Yamagishi, Tadanori Yamochi, Sanaz Firouzi, Haruka Momose, Takuo Mizukami, Kazuya Takizawa, Kumiko Araki, Kazuo Sugamura, Kazunari Yamaguchi, Toshiki Watanabe, Isao Hamaguchi

Quantitative PCR (qPCR) for human T-lymphotropic virus 1 (HTLV-1) is useful for measuring the amount of integrated HTLV-1 proviral DNA in peripheral blood mononuclear cells. Many laboratories in Japan have developed different HTLV-1 qPCR methods. However, when six independent laboratories analyzed the proviral load of the same samples, there was a 5-fold difference in their results.

Source: Journal of Clinical Microbiology vol.53 no.2 , February 2015

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Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroids

Hideyuki Suenaga, Katsuko S. Furukawa, Yukako Suzuki, Tsuyoshi Takato, Takashi Ushida

Mesenchymal stem cell (MSC) condensation contributes to membrane ossification by enhancing their osteodifferentiation. We investigated bone regeneration in rats using the human bone marrow-derived MSC-spheroids prepared by rotation culture, without synthetic or exogenous biomaterials.

Source: Journal of Materials Science: Materials in Medicine November 2015

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Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery

Jianbin Wang , Joshua J. DeClercq, Samuel B. Hayward, Patrick Wai-Lun Li, David A. Shivak, Philip D. Gregory, Gary Lee, and Michael C. Holmes

The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise in recent clinical trials. Nuclease-driven genome editing provides a method in which to precisely target genetic changes to further enhance T cell function in vivo.

Source: Nucl. Acids Res. (2015)doi: 10.1093/nar/gkv1121 First published online: November 2, 2015

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Suppression of cancer relapse and metastasis by inhibiting cancer stemness

Youzhi Li, Harry A. Rogoff, Sarah Keates, Yuan Gao, Sylaja Murikipudi, Keith Mikule, David Leggett, Wei Li, Arthur B. Pardee, and Chiang J. Li

Here we show that BBI608, a small molecule identified by its ability to inhibit gene transcription driven by Stat3 and cancer stemness properties, can inhibit stemness gene expression and block spherogenesis of or kill stemness-high cancer cells isolated from a variety of cancer types. Moreover, cancer relapse and metastasis were effectively blocked by BBI608 in mice.

Source: PNAS | February 10, 2015 | vol. 112 | no. 6 | 1839–1844

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