HIV-1 is Restricted Prior to Integration in Primary Cord-Derived Human CD34+ Cells
Daniel O. Griffin and Stephen Goff
JVI Accepted Manuscript Posted Online 20 May 2015
Certain cells have the ability to block retroviral infection at specific stages of the viral cycle by the activities of well-characterized factors and transcriptional silencing machinery. Infection of murine stem cells (MSCs) by the murine leukemia viruses (MLVs) is profoundly blocked post-integration by transcriptional silencing.
Human Mesenchymal Stromal/Stem Cell-Mediated Bone Marrow Organization
Yasuo Miura , Tatsuo Ichinohe, Taira Maekawa
Japanese Journal of Transfusion and Cell Therapy Vol. 61 (2015) No. 5 p. 489-490
Mesenchymal stromalstem cells (MSCs) in bone marrow differentiate into cellular components of the hematopoietic cell niche, including osteogenic cells. A human MSC transplantation into mice (xenotransplantation model) is one of the best available in vivo models to recapitulate MSC-mediated bone and bone marrow organization.
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn & Matthew H Porteus
Nature Biotechnology 33, 985–989 (2015), Published online 29 June 2015
CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34+ hematopoietic stem and progenitor cells.
Agonist antibody that induces human malignant cells to kill one another
Kyungmoo Yeaa , Hongkai Zhangb , Jia Xieb , Teresa M. Jonesb , Chih-Wei Linb , Walter Francesconic , Fulvia Bertond , Mohammad Fallahie , Karsten Sauerb,f, and Richard A. Lernerb
Proceedings of the National Academy of Sciences of the United States of America, Contributed by Richard A. Lerner, September 25, 2015
An attractive, but as yet generally unrealized, approach to cancer therapy concerns discovering agents that change the state of differentiation of the cancer cells. Recently, we discovered a phenomenon that we call “receptor pleiotropism” in which agonist antibodies against known receptors induce cell fates that are very different from those induced by the natural agonist to the same receptor
A novel antibody–drug conjugate targeting SAIL for the treatment of hematologic malignancies
SY Kim , J-W Theunissen , J Balibalos , S Liao-Chan , MC Babcock , T Wong , B Cairns , D Gonzalez , EH van der Horst , M Perez , Z Levashova , L Chinn , JA D‘Alessio , M Flory , A Bermudez , DY Jackson , E Ha , J Monteon , MF Bruhns , G Chen, and T-S Migone
Citation: Blood Cancer Journal (2015) 5, e316; doi:10.1038/bcj.2015.39 Published online 29 May 2015
Although several new therapeutic approaches have improved outcomes in the treatment of hematologic malignancies, unmet need persists in acute myeloid leukemia (AML), multiple myeloma (MM) and non-Hodgkin’s lymphoma.
Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells
Marahaini Musa, M.Sc,1 Khadijah Mohd Ali, B.Sc,1 Thirumulu Ponnuraj Kannan, Ph.D,1,2,* Ahmad Azlina, Ph.D,1 Nor Shamsuria Omar, M.Sc,1,3 Anil Chatterji, Ph.D,4 and Khairani Idah Mokhtar, Ph.D5
Cell J. 2015 Summer; 17(2): 253–263.
This is an in vitro experimental study. PVF samples were collected from horseshoe crabs from beaches in Malaysia and the crude extract was prepared. DPSCs were treated with different concentrations of PVF crude extract in an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (cytotoxicity test). We choose two inhibitory concentrations (IC50 and IC25) and two PVF concentrations which produced more cell viability compared to a negative control (100%) for further tests.
Potential Small Guide RNAs for tRNase ZL from Human Plasma, Peripheral Blood Mononuclear Cells, and Cultured Cell Lines
Sho Ninomiya , Mitsuoki Kawano, Takashi Abe , Tatsuya Ishikawa , Masayuki Takahashi , Masato Tamura , Yoshiaki Takahashi , Masayuki Nashimoto
PLoS ONE Published March 2, 2015
Several pieces of evidence suggest that small RNA degradation products together with tRNase ZL appear to form another layer of the whole gene regulatory network. The degraded RNA such as a 5′-half-tRNA and an rRNA fragment function as small guide RNA (sgRNA) to guide the enzyme to target RNA.
A phase 1b/2 study of combination vosaroxin and cytarabine in patients with relapsed or refractory acute myeloid leukemia
Jeffrey E. Lancet, Gail J. Roboz, Larry D. Cripe, Glenn C. Michelson, Judith A. Fox, Richard D. Leavitt, Tianling Chen, Rachael Hawtin, Adam R. Craig, Farhad Ravandi, Michael B. Maris, Robert K. Stuart, and Judith E. Karp
Haematologica. 2014; 99:xxx doi:10.3324/haematol.2014.114769
Vosaroxin is a first-in-class anticancer quinolone derivative that intercalates DNA and inhibits topoisomerase II. This study assessed the safety and tolerability of vosaroxin plus cytarabine in patients with relapsed/refractory acute myeloid leukemia. Escalating vosaroxin doses (10-minute infusion; 10-90 mg/m2; days 1, 4) were given in combination with cytarabine on 1 of 2 schedules: schedule A (24-hour continuous intravenous infusion, 400 mg/m2/day, days 1-5) or schedule B (2-hour intravenous infusion,1 g/m2/day, days 1-5).
TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins
Ju Shi, Eileen L. Rose, Andrew Singh, Sami Hussain, Nancy E. Stagliano, Graham C. Parry, and Sandip Panicker
J Shi, EL Rose, A Singh, S Hussain… - 2014 - dnl-epi02.prodno.osl.basefarm.net
Here we test the effects of TNT003, amouse monoclonal antibody targeting the CP-specific serine protease C1s, on CP activity induced by cold agglutininson human RBCs.
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