Identification of TL-Om1, an Adult T-Cell Leukemia (ATL) Cell Line, as Reference Material for Quantitative PCR for Human T-Lymphotropic Virus 1

Madoka Kuramitsu, Kazu Okuma, Makoto Yamagishi, Tadanori Yamochi, Sanaz Firouzi, Haruka Momose, Takuo Mizukami, Kazuya Takizawa, Kumiko Araki, Kazuo Sugamura, Kazunari Yamaguchi, Toshiki Watanabe, Isao Hamaguchi

Journal of Clinical Microbiology vol.53 no.2 , February 2015

Quantitative PCR (qPCR) for human T-lymphotropic virus 1 (HTLV-1) is useful for measuring the amount of integrated HTLV-1 proviral DNA in peripheral blood mononuclear cells. Many laboratories in Japan have developed different HTLV-1 qPCR methods. However, when six independent laboratories analyzed the proviral load of the same samples, there was a 5-fold difference in their results.

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