Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery

Jianbin Wang , Joshua J. DeClercq, Samuel B. Hayward, Patrick Wai-Lun Li, David A. Shivak, Philip D. Gregory, Gary Lee, and Michael C. Holmes

Nucl. Acids Res. (2015)doi: 10.1093/nar/gkv1121 First published online: November 2, 2015

The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise in recent clinical trials. Nuclease-driven genome editing provides a method in which to precisely target genetic changes to further enhance T cell function in vivo.

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