Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells


Marahaini Musa, M.Sc,1 Khadijah Mohd Ali, B.Sc,1 Thirumulu Ponnuraj Kannan, Ph.D,1,2,* Ahmad Azlina, Ph.D,1 Nor Shamsuria Omar, M.Sc,1,3 Anil Chatterji, Ph.D,4 and Khairani Idah Mokhtar, Ph.D5

Cell J. 2015 Summer; 17(2): 253–263.

This is an in vitro experimental study. PVF samples were collected from horseshoe crabs from beaches in Malaysia and the crude extract was prepared. DPSCs were treated with different concentrations of PVF crude extract in an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (cytotoxicity test). We choose two inhibitory concentrations (IC50 and IC25) and two PVF concentrations which produced more cell viability compared to a negative control (100%) for further tests.

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