Stem/Progenitor Cells

Stem/Progenitor Cells

Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells

Marahaini Musa, M.Sc,1 Khadijah Mohd Ali, B.Sc,1 Thirumulu Ponnuraj Kannan, Ph.D,1,2,* Ahmad Azlina, Ph.D,1 Nor Shamsuria Omar, M.Sc,1,3 Anil Chatterji, Ph.D,4 and Khairani Idah Mokhtar, Ph.D5

Cell J. 2015 Summer; 17(2): 253–263.

This is an in vitro experimental study. PVF samples were collected from horseshoe crabs from beaches in Malaysia and the crude extract was prepared. DPSCs were treated with different concentrations of PVF crude extract in an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (cytotoxicity test). We choose two inhibitory concentrations (IC50 and IC25) and two PVF concentrations which produced more cell viability compared to a negative control (100%) for further tests.

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn & Matthew H Porteus

Nature Biotechnology 33, 985–989 (2015), Published online 29 June 2015

CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency in human primary T cells and CD34+ hematopoietic stem and progenitor cells.

HIV-1 is Restricted Prior to Integration in Primary Cord-Derived Human CD34+ Cells

Daniel O. Griffin and Stephen Goff

JVI Accepted Manuscript Posted Online 20 May 2015

Certain cells have the ability to block retroviral infection at specific stages of the viral cycle by the activities of well-characterized factors and transcriptional silencing machinery. Infection of murine stem cells (MSCs) by the murine leukemia viruses (MLVs) is profoundly blocked post-integration by transcriptional silencing.

Suppression of cancer relapse and metastasis by inhibiting cancer stemness

Youzhi Li, Harry A. Rogoff, Sarah Keates, Yuan Gao, Sylaja Murikipudi, Keith Mikule, David Leggett, Wei Li, Arthur B. Pardee, and Chiang J. Li

PNAS | February 10, 2015 | vol. 112 | no. 6 | 1839–1844

Here we show that BBI608, a small molecule identified by its ability to inhibit gene transcription driven by Stat3 and cancer stemness properties, can inhibit stemness gene expression and block spherogenesis of or kill stemness-high cancer cells isolated from a variety of cancer types. Moreover, cancer relapse and metastasis were effectively blocked by BBI608 in mice.

Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium

To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).

Braz. J. Oral Sci. vol.14 no.2 Piracicaba Apr./June 2015

To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).

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