Lymphoid Cells

Lymphoid Cells

Epigenetic remodeling in B-cell acute lymphoblastic leukemia occurs in two tracks and employs embryonic stem cell-like signatures

Seung-Tae Lee, Marcus O. Muench, Marina E. Fomin, Jianqiao Xiao, Mi Zhou, Adam de Smith, Jose I. Martın-Subero, Simon Heath, E. Andres Houseman, Ritu Roy, Margaret Wrensch, John Wiencke, Catherine Metayer, and Joseph L. Wiemels

Nucleic Acids Research, 2015, published online February 17, 2015

We investigated DNA methylomes of pediatric Bcell acute lymphoblastic leukemias (B-ALLs) using whole-genome bisulfite sequencing and highdefinition microarrays, along with RNA expression profiles. Epigenetic alteration of B-ALLs occurred in two tracks: de novo methylation of small functional compartments and demethylation of large intercompartmental backbones.

Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery

Jianbin Wang , Joshua J. DeClercq, Samuel B. Hayward, Patrick Wai-Lun Li, David A. Shivak, Philip D. Gregory, Gary Lee, and Michael C. Holmes

Nucl. Acids Res. (2015)doi: 10.1093/nar/gkv1121 First published online: November 2, 2015

The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise in recent clinical trials. Nuclease-driven genome editing provides a method in which to precisely target genetic changes to further enhance T cell function in vivo.

Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens

Wei Liao, Gwen Jordaan, Phillipp Nham, Ryan T. Phan, Matteo Pelegrini, and Sanjai Sharma

BioMed Central, October 16, 2015

To determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.

Central memory CD4+ T cells are preferential targets of double infection by HIV-1

Aiman A. Haqqani , Samantha L. Marek, Jagadish Kumar, Miles Davenport, Heng Wang, and John C. Tilton

Virology Journal 2015 12:184 Published: 11 November 2015

Template switching between two distinct HIV-1 RNA genomes during reverse transcription gives rise to recombinant viruses that greatly expand the genetic diversity of HIV-1 and have adverse implications for drug resistance, immune escape, and vaccine design. Virions with two distinct genomes are produced exclusively from cells infected with two or more viruses, or ‘doubly infected’ cells.

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