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Peripheral Blood CD19+ B Cells

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Untouched NPB-CD19+ B Cells are negatively isolated from mononuclear cells using an indirect immunomagnetic labeling system.

Positively Selected NPB-CD19+ B Cells are directly isolated from mononuclear cells using immunomagnetic CD19 MicroBeads.

A '-P' in catalog # indicates positively-selected cells.
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ItemLegacy Catalog #FormatSelection MethodSizePriceQuantity 
LP, CR, CD19+, NS, 10MPB010FCryopreserved Negative10 million
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LP, FR, CD19+, NS, 10MPB010Fresh Negative10 million
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LP, CR, CD19+, PS, 10MPB010-P-FCryopreserved Positive10 million
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Overview

Additional Information

Untouched NPB-CD19+ B Cells are negatively isolated from mononuclear cells using an indirect immunomagnetic labeling system.

Positively Selected NPB-CD19+ B Cells are directly isolated from mononuclear cells using immunomagnetic CD19 MicroBeads.

A '-P' in catalog # indicates positively-selected cells.

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Donor Criteria

Age18-55 years old
Weight140-280 lbs
Screened before donation
  • HIV (HIV 1 & 2 Ab)
  • HBV (Surface Antigen HbsAg)
  • HCV (HCVAb)
Donation Frequency
  • Minimum 4 months between donations
  • Half Pak: 2x donations 6-12 weeks apart
Donors with any of the following will be excluded from donatingPregnancy
History of heart, lung, liver, or kidney disease
History of asthma
Blood and bleeding disorders including sickle cell disease
Neurologic disorders
Autoimmune disorders
Cancer
Diabetes
Other CriteriaMust be in general good health
Must have adequate peripheral veins
Complete Blood Count lab test must meet protocol specs
Required to sign procedure-specific consent form

Applications/Publications

Applications

  • Generating antibody libraries
  • Co-culture with T helper cells or cytotoxic lymphocytes (CTL)

To Study

  • B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
  • Antigen presenting cell (APC) signal transduction and antigen presentation
  • Antigen presenting cell (APC) signal transduction and antigen presentation
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