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Peripheral Blood CD14+ Monocytes

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Untouched NPB-CD14+ Monocytes are indirectly isolated from mononuclear cells using an immunomagnetic labeling system. Positively-Selected NPB-CD14+ Monocytes are directly isolated from mononuclear cells using an immunomagnetic CD14 MicroBead labeling system.  A '-P' in catalog # indicates positively-selected cells.

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ItemLegacy Catalog #FormatSelection MethodSizePriceQuantity 
LP, CR, CD14+, NS, 10MPB011FCryopreserved Negative10 million
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LP, FR, CD14+, NS, 10MPB011Fresh Negative10 million
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LP, CR, CD14+, PS, 50MPB011-P-F-1Cryopreserved Positive50 million
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LP, FR, CD14+, PS, 10MPB011-P-1Fresh Positive10 million
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LP, FR, CD14+, PS, 50MPB011-P-2Fresh Positive50 million
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Overview

Additional Information

Untouched NPB-CD14+ Monocytes are indirectly isolated from mononuclear cells using an immunomagnetic labeling system. Positively-Selected NPB-CD14+ Monocytes are directly isolated from mononuclear cells using an immunomagnetic CD14 MicroBead labeling system.  A '-P' in catalog # indicates positively-selected cells.



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Donor Criteria

Age18-55 years old
Weight140-280 lbs
Screened before donation
  • HIV (HIV 1 & 2 Ab)
  • HBV (Surface Antigen HbsAg)
  • HCV (HCVAb)
Donation Frequency
  • Minimum 4 months between donations
  • Half Pak: 2x donations 6-12 weeks apart
Donors with any of the following will be excluded from donatingPregnancy
History of heart, lung, liver, or kidney disease
History of asthma
Blood and bleeding disorders including sickle cell disease
Neurologic disorders
Autoimmune disorders
Cancer
Diabetes
Other CriteriaMust be in general good health
Must have adequate peripheral veins
Complete Blood Count lab test must meet protocol specs
Required to sign procedure-specific consent form

Applications/Publications

Applications

  • Generate monocyte-derived M1 and M2 macrophages
  • Generate monocyte-derived DCs

To Study

  • Dendritic cell differentiation and maturation
  • Toll-like receptor and pattern recognition receptors (PRR)
  • Phagocytosis
  • Metabolism mechanisms
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