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Mobilized Peripheral Blood Leuko Pak

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AllCells has been a pioneer in the use of G-CS F (Granulocyte-Colony Stimulating Factor) stimulation in normal healthy donors since 1998. The protocols in use for our Mobilized Peripheral Blood (MPB) products have been approved by an IRB and are overseen by physicians. In addition AllCells offers Plerixafor (Mozobil®) or a combination of Plerixafor (Mozobil®) and Neupogen mobilization of stem cells from healthy donors.

One-Collection Mozobil MPB-Leuko Pak (Moz.A)- This product is obtained by a 240 μg/kg/day of Mozobil injection for one day and apheresis 9-11 hours post injection.

One-Collection G-CSF MPB-Leuko Pak (Reg.G)- This product is obtained by 10 μg/kg/day of G-CSF injections for 5 days and apheresis on Day 6. (CUSTOM - INQUIRE REGARDING AVAILABILITY AND PRICE)

Two-Collection G-CSF MPB-Leuko Pak (Reg.F)- This product is obtained by 10 μg/kg/day of G-CSF injections for 5 days and apheresis on Day 5 and Day 6 (indicate which day's collection to receive as 1 apheresis bag).

Two-Collection G-CSF & Mozobil MPB-Leuko Pak (Reg.H)- This product is obtained by 10 μg/kg/day of G-CSF injections for 5 days and 240 μg/kg/day of Mozobil on 2 days (day 4 and 5) and apheresis on Day 5 and Day 6 (indicate which day's collection to receive as 1 apheresis bag).

Leuko Pak Cell Number Ranges*
MNC Avg. Cell Number: 2.3x1010 Range: 0.9 to 5.6x1010
CD34+ Avg. Cell Number: 1.86x108 Range: 0.6 to 4.8x108

*Please note that average cell numbers and ranges are for reference purposes only. AllCells cannot guarantee exact cell numbers due to donor-to-donor variability.

Contact Us: Mon-Fri 7:30am to 6:00pm PST    510.726.2700    orders@allcells.com
ItemLegacy Catalog #FormatProduct nameSizePriceQuantity 
mLP, MozA, FR, 1 BagmPB025-Moz.AFresh Mobilized Leuko Pak, Fresh, Mozobil(R) 20mg/mL, 1.2mL Single Dose, 1 Bag1 bag
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mLP, RegF, FR, 1 BagmPB025-Reg.FFresh Mobilized Leuko Pak, Fresh, Neupogen(R) 10mcg/kg/day x 5 days, 1 Bag1 bag
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mLP, RegH, FR, 1 BagmPB026-REG.HFresh Mobilized Leuko Pak, Fresh, Neupogen(R) 10mcg/kg, Mozobil 240 mcg/kg, Apheresis On Day 5 and Day 6, 1 Bag1 bag
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Overview

Additional Information



Customization

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Donor Criteria

Age18-40 years old
Weight140-280 lbs
Screened before donation
  • HIV (HIV 1 & 2 Ab)
  • HBV (Surface Antigen HbsAg)
  • HCV (HCVAb)
Donation Frequency
  • Minimum 12 months between donations
  • 2x lifetime donation maximum
Donors with any of the following will be excluded from donatingPregnancy
History of heart, lung, liver, or kidney disease
History of asthma
Blood and bleeding disorders including sickle cell disease
Neurologic disorders
Autoimmune disorders
Cancer
Diabetes
Other Criteria
  • Must be in general good health
  • Must have adequate peripheral veins
  • Complete Blood Count lab test must meet protocol specs
  • Required to sign procedure-specific consent form

Applications/Publications

Applications

  • Proof of concept studies for allogenic immunotherapy and gene therapy products

To Study

  • Hematopoeisis
  • Aphereisis
  • Mobilization by Neupogen® (G-CSF) or or Mozobil® (Plerixafor) physiology

Related Bioservices

  • ISHAGE
  • CFU

Publications

Optimized Processing of Growth Factor Mobilized Peripheral Blood CD34 Products by Counterflow Centrifugal Elutriation

CHY-ANH TRAN, et al.
Source: Stem Cells Trans Med May 2012 vol. 1 no. 5 422-429

Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor cells

Dolores L Mahmud, et al.
Source: Oncogene (2002) 21, 1556 ± 1562

Na pump isoforms in human erythroid progenitor cells and mature erythrocytes

Joseph F. Hoffman, et al.
Source: www.pnas.orgcgidoi10.1073pnas.222539999

A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology

Ryan E Campbell-Anson. et al.
Source: Journal of Immune Based Therapies and Vaccines 2008, 6:1
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