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Peripheral Blood Monocyte-Derived Dendritic Cells

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NPB-Monocyte Derived Myeloid Dendritic Cells (MoDC) are derived from highly purified monocytes. For seven days, the highly purified monocytes are cultured with 10% Fetal Bovine Serum in the presence of GM-CSF and IL-4. These cells express the following markers: CD11c, CD40, and CD86. These cells lack the expression of CD14.
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Item #Legacy Catalog #FormatSizePriceQuantity 
LP, CR, DCs, Cultured, 1MPB-DC001F Cryopreserved 1 million
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Overview

Additional Information

NPB-Monocyte Derived Myeloid Dendritic Cells (MoDC) are derived from highly purified monocytes. For seven days, the highly purified monocytes are cultured with 10% Fetal Bovine Serum in the presence of GM-CSF and IL-4. These cells express the following markers: CD11c, CD40, and CD86. These cells lack the expression of CD14.

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Donor Criteria

Age18-55 years old
Weight140-280 lbs
Screened before donation
  • HIV (HIV 1 & 2 Ab)
  • HBV (Surface Antigen HbsAg)
  • HCV (HCVAb)
Donation Frequency
  • Minimum 4 months between donations
  • Half Pak: 2x donations 6-12 weeks apart
Donors with any of the following will be excluded from donatingPregnancy
History of heart, lung, liver, or kidney disease
History of asthma
Blood and bleeding disorders including sickle cell disease
Neurologic disorders
Autoimmune disorders
Cancer
Diabetes
Other CriteriaMust be in general good health
Must have adequate peripheral veins
Complete Blood Count lab test must meet protocol specs
Required to sign procedure-specific consent form

Applications/Publications

Applications

  • Mixed lymphocyte Reaction (MLR) assays

To Study

  • Innate immunity signaling and activation pathways
  • Toll-like receptors (TLR) and other Pattern Recognition Receptors (PRR)
  • Phagocytosis
  • Antigen processing and presentation
Video & Technical Resources
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