First, the mouse spleen is dissociated into a single cell suspension. Next, the spenocytes are enriched by density separation. The rat splenocytes can be used to isolate CD4+ T cells, CD8+ T cells, and CD19+ B cells using specific immunomagnetic methods.
Mouse Splenocytes can be used to study:
- Growth factor and cytokine release from lymphocytes as well as monocytes and Kupffer cells
- Function and mechanism of normal and diseased models in the reticuloendothelial system
- Homing and processing of red blood cells
- Antigen-recognition conditioning and proliferation of lymphocytes and monoctyes
