First, the SD rat spleen is mechanically dissociated into a single cell suspension. The splenocytes are enriched by density separation. The rat splenocytes can be used to isolate CD4+ T cells, CD8+ T cells, and CD45R+ B cells, using specific immunomagnetic methods.
Rat Splenocytes can be used to study:
- Growth factor and cytokine release from lymphocytes, monocytes and Kupffer cells
- Antigen-recognition conditioning and proliferation of lymphocytes and monocytes
- Reserve mechanisms for red blood cells and monocytes
