Bone Marrow

Background

Bone Marrow (BM) contains hematopoietic stem/progenitor cells, which are self-renewing, proliferating, and differentiating into multi-lineage blood cells. Multipotent, non-hematopoietic stem cells, such as mesenchymal stem cells, can be isolated from human BM as well. These non-hematopoeitic, mesenchymal stem cells are capable of both self-renewal and differentiation into bone, cartilage, muscle, tendons, and fat. BM is drawn into a 60cc syringe containing heparin (80 U/mL of BM) from the posterior iliac crest, 25mL/site, from a maximum of four sites. Whole BM products are diluted with PBS.

DONOR CRITERIA

• Age: 18-55 years old
• Weight: >110 lbs for 25mL and 50mL donations
• Weight: >130 lbs for 100mL donations
• CBC lab tests must meet protocol specifications.
• Donors cannot be pregnant.
• Donors are screened for HIV, Hepatitis B and Hepatitis C before donation.
• Donors must be in general good health. Donors with any of the following will be excluded from donating:
  • History of heart, lung, liver, kidney disease (including asthma)
  • Blood and bleeding disorders, including sickle cell disease
  • Neurologic disorders
  • Cancer
  • Diabetes
  • Positive: HIV, HbsAg, HCAb
• Donors must have adequate peripheral veins for apheresis.
• Donors are required to sign a procedure-specific consent form prior to the collection procedure.
• All consents and protocols have been approved by an independent biomedical review board.