AllCells specializes in immunoassays as part of our Bioservices divison. Immunoassays are a robust method of detecting cellular response and assessing drug efficacy through protein expression/secretion. These assays have industry applications in autoimmunity, transplantation, vaccine development, therapeutic development, and many more. AllCells provides immunoassays services including ELISA, ELISpot, luminex assay, multiplex assay, immunoassay screening and Human Donor HLA Typing amongst others.
Enzyme-linked immunosorbent assay, or ELISA, is a quantitative assay that can be used to characterize expression levels of soluble proteins. This assay features a high throughput, scalable, easily-read platform. Due to its flexibility, ELISA can be applied to numerous targets depending on your specific requirements.
Anti-CD40-induced IgG secretion from plasma cells of 3 healthy donors
Figure A. Plasma B cells isolated from peripheral blood of 3 human donors were stimulated with (blue bars) or without (red bars, undetectable) anti-CD40. Supernatants were assayed for lgG by ELISA. B. Standard curve showing range of IgG protein detection.
Enzyme-linked immunospot assay, or ELISpot, is a colorimetric assay which enables quantitative, single cell analysis of protein expression levels. Due to its high sensitivity and reproducibility, the ELISpot is an industry standard in antigenic response in the form of cytokine secretion.
T cell proliferation assay
Labeling of T cells with the fluorescent dye 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) allows for quantitative phenotyping of T cell division in response to antigen or other stimuli by flow cytometric methods. T cell proliferation assays are essential for peptide epitope mapping of naïve T cells and for characterizing full protein immunogenicity in DC-T cell assays.
Proliferation of CD4+ and CD8+ primary human T cells
Figure. CD4+ (A) and CD8+ (B) T cells were isolated from peripheral human blood and labeled with 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). Labeled cells were cultured 7d with or without a stimulatory antibody cocktail. Fluorescence was measured by flow cytometry.
Luminex (single, multiplex)
The Luminex Bead Array System allows for a high throughput, multi-target analysis of cytokines, proteins, and nucleic acids for researchers. The system is important for many clinical and research applications.
Human Donor HLA Typing
HLA (Human Leukocyte Antigen) Typing of donors is an important aspect of research being conducted within inflammatory, immune responses pertaining to antigen presentation. AllCells Bioservices provides HLA Typing of our donors for specific A2, DRB4 loci. We offer this service based on the specific research criteria you may have. Please inquire with us today about our HLA Typing capabilities and how we may assist with your project needs.
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