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AllCells
AllCells

Flow Cytometry Services

Flow Cytometry Services

AllCells utilizes 20+ years of flow cytometry service experience and state-of-the-art equipment to provide customized research solutions. We offer Multivariable Flow Cytometry Analysis with up to 7 colors simultaneously using our FACSCalibur® and FACSCanto II® machines. Our services include immunophenotyping of intracellular and extracellular molecules, FACS analysis, DNA analysis, CD34 enumeration and leukocyte characterization using ISHAGE guidelines.

Our Immunophenotyping services can be applied to cellular characterization of both intracellular and extracellular molecules using fluorescently-labeled antibodies. These include but are not limited to MHC/HLA Typing, cancer cell analysis (focusing on hematological malignancies), and other custom analyses. 

Our DNA analysis services can provide information on the percentage of cells in each phase of the cell cycle. This output can be used in drug screening applications, inhibition, proliferation, senescence, etc.

Flow-cytometric
enumeration of CD34+ hematopoietic stem and progenitor cells (HSCs) is widely used for evaluation of graft adequacy of peripheral blood and bone marrow stem cell grafts. The International Society of Hematotherapy and Graft Engineering (ISHAGE) have created guidelines for a gating strategy that results in replicable standards for stem and progenitor cell enumeration. AllCells Bioservices adheres to ISHAGE guidelines for characterization of leukocytes and quantification of CD34+ cells.
 
Fluorescence-activated Cell Sorting (FACS analysis) utilizes the principles of flow cytometry to identify populations of cells of interest. Cells can be electrically charged and sorted into highly purified populations. AllCells FACS flow cytometry services are capable of identifying and sorting cell populations using up to 11 colors.

Our Bioservices team can provide customized applications of the system to accelerate your research. In addition to flow cytometry analysis we also provide a number of other services including: immunoassays, stem cell assays, protein analysis, genotyping, and tissue culture services. Inquire with us today by filling out our technical support form at
www.allcells.com/technical-support/.


Case Study
: Extracellular LSK markers were placed on mouse bone marrow cells. LSK markers are an indication of mouse hematopoietic cells (HSCs) in the mouse bone marrow.

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